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Chromatography
Generon Catalogue | Chromatography
| Proteus Mini Purification Spin Columns |  | Generon’s Proteus mini purification spin columns are designed for small scale protein purifications such as those required for expression trials, solubility determination tests, screening, tittering and scouting studies. The columns fit all standard microfuges and allow you to process multiple samples in parallel. |
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| Single StEP Empty Chromatograhy Columns |  | Thompson’s Single StEP Empty Columns allow you the opportunity to fill different sized columns with resins for protein purification applications. The sample is mixed with the resin and batch incubated. The columns can then be attached to an FPLC or peristaltic pump and the purified protein can then eluted with a gradient. |
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| 1-Step Batch Spin Columns |  | The innovative Proteus ‘1-Step Batch’ Spin Column allows you to batch mix your filtered or unclarified sample with the resin of your choice and then wash and elute your purified and concentrated protein in a small volume. There is no resin carry-over to a subsequent downstream analyses, no messy transfer of samples/resin slurries and negligible hold-up volume. As the device is contained, it is ideal for handling bio-hazardous samples and samples from infectious sources. |
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| Ni Superflow Resin |  | The Gold Standard for His-tagged Protien Purification. Generon's Nickel Superflow is a tetra-dentate chelator like Ni-NTA Superflow. It can withstand much higher pressures than Sepharose resins which makes Generon's Ni-Superflow resin much more capable for column It also have excellent tolerance to reducing agents such as DTT or 2-ME. This resin is regarded as one of the best Ni- IMAC resins on the market for batch and column purification. |
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| Sartorius Adenovirus Purification Kits |  | Recombinant adenovirus vectors are the preferred method for a wide range of gene delivery applications. The AdenoPACK adenovirus purification and concentration kits offer researchers who need to recover up to 3 x 10^13 purified recombinant adenovirus particles for in vitro transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures in only two hours. These straight forward kits replace time-consuming and labour-intensive 48 hour CsCl density gradients. |
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| Rapid Lentivirus Purification Kits | | The LentiSELECT 40, 500 and 1000 lentivirus purification and concentration kit offers researchers who need to recover up to 4–5 x 10^9 purified recombinant lentivirus particles for in vitro transfection a fast, safe and easy to use solution. Like the AdenoPACK kits, they include all reagents and devices necessary for purification and concentration of VSV-G pseudotyped lentivirus from HEK293 cell cultures. |
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| Rapid Chromatography Columns |  | Proteus spin columns are convenient and easy to use. Kits are supplied with powerful, affinity and single step spin columns, clear and unambiguous protocols, a detailed reference handbook, desalting/buffer exchange columns and ready-to-use buffers. Multiple samples can be purified at the same time with high reproducibility for rapid purification of your antibodies or 6xHis-tagged recombinant proteins. These spin columns contain a unique flow regulator that imparts flow rate control during the centrifugation step. Sample loading is performed at a flow rate close to 1 ml/min. In this way, you get the speed of a spin column and the purities and yields of a gravity column. The high capture efficientcies generate typical capacities of 20-30 mg IgG or His-tagged protein in under 50 mins. |
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| Loose Resin |  | We offer premium Protein A, Protein G agarose and Ni-agarose resin for single step affinity purification of polyclonal/monoclonal antibodies and His-tagged tagged proteins. The Ni-agarose resin offer excellent value with prices and performance unparalleled. |
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| Glutathione Superflow Resin | | SuperGlu (Glutathione Superflow) is designed for rapid one-step purification of glutathione S-transferase-tagged proteins from bacteria, yeast, insect and mammalian cultures. Glutathione is covalently coupled to Superflow matrix that provides a high chemical and physical stability and excellent flow rates. SuperGlu is made in the particle size range of 60-160 µm and has a dynamic binding capacity of 10 mg recombinant GST/ml resin. Glutathione Superflow has excellent GST-tagged protein binding characteristics and unlike Glutathione Sepharose, it can withstand pressures up to 120-140 psi. |
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Generon Catalogue | Chromatography
Generon Ltd, 12 Rawcliffe House, Howarth Road, Maidenhead, Berkshire, SL6 1AP Tel: +44 (0)1753 866 511 Fax: +44 (0)1753 864 942
