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Protein Expression, Purification and Characterization Service

Generon Catalogue |  Protein Expression, Purification and Characterization Service

Cloning Service  


Price: £800.00 (Excluding VAT at 17.5%)
Quantity:  

Cloning (<1kb cDNA insert) from accession number- using either a cDNA library or an image clone as a template. We will design oligonucleotide primers to amplify the gene of interest and then clone this into the desired vector of choice. Constructs will be fully verified by DNA Sequencing. Prices for larger inserts available upon request.
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Bacteria Phase I  


Price: £550.00 (Excluding VAT at 17.5%)
Quantity:  

A small scale pilot study which includes: bacterial transformation, expression test using both IPTG and auto-induction methods, solubility testing, SDS-PAGE gel analyses and Western blotting.
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Bacteria Phase II  


Price: £800.00 (Excluding VAT at 17.5%)
Quantity:  

1 L scale up using the optimised conditions elucidated in Phase I to include protein expression, affinity purification, tag removal, second step purification (if required) and SDS-PAGE analyses of all protein fractions. Affinity tag will be removed if required by the appropriate enzyme digestion (eg. thrombin, TEV etc) and cleaved proteins purified by gel filtration or anion exchange chromatography.
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Insect Phase I  


Price: £800.00 (Excluding VAT at 17.5%)
Quantity:  

A small scale pilot study which includes: co-transfection of baculovirus DNA and transfer plasmid into Sf9 cells, P1 stock virus amplification, test infection in 6-well dishes using both Sf9 and Hi5 cell lines, at T48 and T72 hours with five concentrations of recombinant virus plus an infected control. SDS-PAGE analyses of cell fractions (total/soluble/pellet/secreted) and Western blot analysis.
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Insect Phase II  


Price: £1,010.00 (Excluding VAT at 17.5%)
Quantity:  

0.5-1L scale up using the optimized conditions elucidated in Phase I to include: amplification to P2 and P3 of virus stocks, infection of 0.5-1L of cells, protein affinity purification, tag removal, second step purification (if required) and SDS-PAGE analyses of all protein fractions. Affinity tag will be removed if required by the appropriate enzyme digestion (eg thrombin, TEV etc) and cleaved proteins purified by gel filtration or anion exchange chromatography.
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Mammalian Phase I  


Price: £600.00 (Excluding VAT at 17.5%)
Quantity:  

A small scale pilot study which includes: transfection into HEK293 EBNA cells and antibiotic selection of transfected cells. SDS-PAGE analyses of cell fractions (total/soluble/pellet/secreted) and Western blot analysis.
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Mammalian Phase II  


Price: £1,200.00 (Excluding VAT at 17.5%)
Quantity:  

0.5-1 L scale up using the optimised conditions elucidated in Phase I to Include growth of 0.5-1L culture, affinity purification, tag removal, second step purification (if required) and SDS-PAGE analyses of all protein fractions. Affinity tag will be removed if required by the appropriate enzyme digestion (eg. thrombin, TEV etc) and cleaved proteins purified by gel filtration or anion exchange chromatography.
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Mammalian Biomolecular Analysis  


Price: £400.00 (Excluding VAT at 17.5%)
Quantity:  

Analytical characterization ensures the identity, purity, structural and conformational integrity of a protein. We perform a number of routine protein analyses throughout all project stages. We can also offer the following analyses for an additional charge: CD-spectroscopy, light scattering and mass spectrometry.
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Generon Catalogue |  Protein Expression, Purification and Characterization Service

Generon Ltd, 12 Rawcliffe House, Howarth Road, Maidenhead, Berkshire, SL6 1AP Tel: +44 (0)1753 866 511 Fax: +44 (0)1753 864 942

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