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Rapid Chromatography Columns
Generon Catalogue | Chromatography | Rapid Chromatography Columns
| Proteus IMAC Spin Column Kits |  | The revolutionary Proteus spin columns are convenient and easy to use to purify your His-tagged fusion proteins. Unlike any other centrifugal column, Proteus contains a unique flow regulator, which is integrated in the resin. Flow rate is inextricably linked to the binding capacity of an affinity column. The flow regulator ensures that the column exerts a back pressure, slowing down the flow of the sample through the column. In this way, you achieve the yields and purities of a gravity column with the speed of a spin column. |
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| Proteus Protein A & G Vacuum Column Kits |  | The peristaltic pump collar accessory permits large volumes of dilute samples to be continuously loaded on to the columns without the need of a centrifuge. Residence times between the target protein and the resin matrix can be closely controlled using a peristaltic pump. Higher recoveries are obtained with slower sample loading flow rates. On a per ml sample volume basis, the peristaltic pump allows faster sample loading rates than spin columns. Typical flow rates between 1-10 ml/min are observed. Up to 1,000 fold concentration of the purified protein or antibody can be recovered in the final eluate. |
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| Proteus Protein A & G Spin Column Kits | | A unique aspect of Pro-Chem's invention involves a proprietary flow regulator which is designed to control the flow rate of the sample through the agarose column. The flow regulator substantially increases the yield and purity of the purified target protein or antibody. No other company offers spin columns with the flow regulator and without the flow regulator, binding capacities and purities drop by over 75 % in a spin column. Kits are supplied with powerful, affinity and single step spin columns, clear and unambiguous protocols, a detailed reference handbook, desalting/buffer exchange columns and ready-to-use buffers. Multiple samples can be purified at the same time with high reproducibility for rapid purification of your antibodies. This makes them ideal for checking, titering, method development, scouting, screening and preparative antibody purification. |
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Generon Catalogue | Chromatography | Rapid Chromatography Columns
Generon Ltd, 12 Rawcliffe House, Howarth Road, Maidenhead, Berkshire, SL6 1AP Tel: +44 (0)1753 866 511 Fax: +44 (0)1753 864 942
