The most competitively-priced premium
Glu agarose resin in the market

Glutathione Affinity Superflow™ (SuperGlu) Resin

• Glutathione Superflow
• High GST-tagged proteins binding capacity
• Rapid one-step purification of GST-tagged proteins
• Negligible ligand leakage
• High recovery of product
• High flow-rate
• cGMP manufacturing
• Drug Master File

The Product
SuperGlu (Glutathione Superflow) is designed for rapid one-step purification of glutathione S-transferase-tagged proteins from bacteria, yeast, insect and mammalian cultures. Glutathione is covalently coupled to Superflow matrix that provides a high chemical and physical stability and excellent flow rates. SuperGlu is made in the particle size range of 60-160 µm and has a dynamic binding capacity of 10 mg recombinant GST/ml resin. SuperGlu is stable with all commonly used buffers and reagents including 0.1M NaOH and organic solvents. Removal of the GST tag (a naturally occurring protein with a molecular weight of 26 kDa) can be undertaken while bound to the column or in solution after elution using typical site-specific proteases such as PreScission™ Protease, Factor Xa or Thrombin.

Glutathione Superflow Affinity Resin

Part No:

Description:

Price:

Super Glu 10

Glutathione Superflow Resin (10 ml)

£100.00

Super Glu 25

Glutathione Superflow Resin (25 ml)

£240.00

Super Glu 100

Glutathione Superflow Resin (100 ml)

£850.00

Super Glu 500

Glutathione Superflow Resin (500 ml)

£3,150.00

Specifications

Agarose concentration:
Bead Size:
Ligand density:
Support:
Binding capacity:
Max. pressure:
Max. linear flow-rate:
Max. flow-rate:
pH stability:
Cleaning:
Slurry concentration:
Antimicrobial agent:
Storage condition:
Exclusion limit:⁶
 

6% cross-linked agarose
60-160 µm
32 µmole/ml gel
Superflow 6 matrix
10 mg GST-tagged protein/ml resin
100-120 psi
1,400 cm/hr (using 1.5 x 13 cm column)
40 ml/min (using 1.5 x 15 cm column)
pH 3-12
0.1M NaOH for 30 min
67%
20% ethanol
20% ethanol at 2-8°C
6 x 10⁶
 

Downloads:

Glutathione Superflow Affinity Resin - Data Sheet
100k

Glutathione Superflow Affinity Resin - Material Safety Data Sheet
100k

Figure 1: Showing high quality purification of a GST-tagged protein from E. coli cell lysate

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Lane 1: E. coli expressing GST lysate
Lane 2: Eluted GST-tagged protein from the main competitor G
Lane 3: Eluted GST-tagged protein from SuperGlu
Lane 4: Molecular weight standards