Fast, Accurate and Affordable Protein Estimation

The Bradford kit provides a quick ready-to-use, Coomassie-binding colorimetric method for accurate total protein quantitation. The protocol is simple with the dye-binding process complete in less than 5 min. The colour is stable for 1 hour.

Ready to use formulation: no dilution is required

Rapid colour development

Broad working concentration range: 1-2,000 mg/ml

Compatible with a wide range of chemicals present in the samples: solvents, salts, thiols, reducing, chelating agents and detergents e.g. 0.1% TX-100, 0.1% TX-114, 5% CHAPS, 0.5% NP-40, 0.06% Tween 20, 0.01% Brij 58P etc...
 

bradfordstandard001

Standard Curve obtained using Bradford Reagent, ready-to-use, in a standard assay.

BSA - bovine serum albumin

BCG - bovine gamma globulin

bradfordmicro

Standard Curve obtained using Bradford Reagent, ready-to-use, in a micro-assay.


Generon's Bradford reagent is an improvement over classical Bradford formulations that cannot tolerate alot of chemical contamination of the protein samples. In addition, unlike BCA and UV methods this product tolerates reducing reagents and UV adsorbing chemicals.

Product Features
• High chemical compatible eg solvents, salts, detergents, thiols,reducing agents and chelating agents
• Ready to use (1x)
• Rapid colour development
• Broad working concentration range-1- 2,000 mg/ml.

Bradford Protein Reagent
Brochure (140kb)

Bradford Protein Reagent
Technical Datasheet (65kb)

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Downloads:

Bradford Reagent Kit from Fermentas