The PageRuler Prestained Protein Ladder is a 3-colour protein standard with 10 prestained recombinant prokaryotic proteins covering a wide range molecular weights from 10 to 170 kDa. The ladder contains one orange reference band at 70 kDa and one green reference band at 10 kDa. The PageRuler Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and for approximate sizing of proteins. Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.
• Monitoring protein transfer onto membranes after Western Blotting.
• Sizing of proteins on SDS-polyacrylamide gels and Western blots. Composition
62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol. Recommendations for Loading
Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil. Mix gently, but thoroughly, to ensure that the solution is homogeneous. Load the following volumes of the ladder on SDS-polyacrylamide gel: 10 µl per well for mini gels; 20 µl per well for large gels. Use the same volumes for Western Blotting. Notes:
Each lot of PageRuler Prestained Protein Ladder is calibrated against a precisely sized PageRuler Unstained Protein Ladder in a Tris-glycine gel and the calculated apparent molecular weights are reported in the product's Certificate of Analysis. Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. A separation gel resolves proteins effectively according to their molecular weight. Linear gradient gels are used for resolution of both small and large proteins, while low percentage gels are recommended for analysis of large proteins. In these gels, small proteins migrate with the tracking dyes during electrophoresis. | 
Features
- Sharp and stable colours.
- Two reference bands - orange at ~70 kDa and green at ~10 kDa.
- Ready to load - no need to boil.
- Easy to track on gel.
- Excellent western transfer. Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western Blotting. Storage
Stable at 4°C for up to 3 months. For long term storage, store at -20°C. |
