Protein Purification Kits
Proteus spin columns are convenient and easy to use. Kits are supplied with powerful, affinity and single step spin columns, clear and unambiguous protocols, a detailed reference handbook, desalting/buffer exchange columns and ready-to-use buffers. Multiple samples can be purified at the same time with high reproducibility for rapid purification of your antibodies or 6xHis-tagged recombinant proteins.

The Mini and Midi agarose spin columns are pre-packed and reusable with minimal preparation time and no mess. Complete separation is achieved in less than 20 mins with the Mini and less than 60 mins with the Midi spin columns.
 

Product

Application

Size

Volume

Bind capacity

Chemistry

Protein A

Affinity Chromatography

Mini

0.65 ml

>1 mg

Protein A agarose

Midi

20 ml

20 mg

Protein G

Affinity Chromatography

Mini

0.65 ml

>1 mg

Protein G agarose

Midi

20 ml

20 mg

IMAC

Affinity Chromatography

Mini

0.65 ml

>1 mg

Ni2+ -IDA agarose

Midi

20 ml

20 mg

Static Concentrator Kits

The BJP concentrates urine through the absorption of water by absorbent pads located on the underside of an ultrafiltration membrane. Bence Jones protein will be retained on the membrane surface in the front compartment of the BJP unit because it has a 7.5 kDa MWCO membrane, a molecular weight cut-off far from the molecular weight of Bence Jones protein itself.

BJP concentrators offer a fast, gentle and convenient method to concentrate multiple clinical samples for analyses by electrophoresis and immunofixation. No pressure lines, vacuum pumps or centrifuges are required. The procedure is simple and straightforward. The operator loads the sample into the bench-top concentrator, waits for the sample to concentrate and recovers the protein concentrate with confidence.

A BJP 5 unit filled with 5 ml urine typically takes about 30-40 min for a 100 fold concentration with recoveries of at least 95-98 % BJP. The BJP 20 static concentrator performs a 100 fold concentration of 10 ml urine in 50 min. The hands-off operation allows the operator time to continue with other analyses in a busy laboratory environment while the device is left unattended to concentrate the urine samples. The impermeable dead stop pocket prevents the urine sample from concentrating to dryness.

BJP concentrators enable rapid and easy concentration of samples:

• Up to 50% faster than the leading device
• 20% less expensive than the leading device
• Up to 98% recovery rate
• Never over concentrates
• Easy to fill
• Easy to read
• Easy sample removal
• Choice of formats
 

Product

Volume

Smaple Wells

Membrane

MWCO

Driving Force

BJP 5

0.5 - 5 ml

1

PES

7.5 kDa

Static absorption

BJP 10

1 - 10 ml

1

PES

7.5 kDa

Static absorption

BJP20

2 - 20 ml

1

PES

7.5 kDa

Static absorption

BJP5/40

0.5 - 5 ml

8

PES

7.5 kDa

Static absorption

BJP10/40

1 - 10 ml

8

PES

7.5 kDa

Static absorption