Fast and easy to use kits for Adenovirus Purification
Recombinant adenovirus vectors are the preferred method for a wide range of gene delivery applications. The AdenoPACK adenovirus purification and concentration kits offer researchers who need to recover up to 3 x 10¹³ purified recombinant adenovirus particles for in-vitro transfection a fast, safe and easy to use solution. The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures in only two hours. These straight forward kits replace time-consuming and labor-intensive 48 hour CsCl density gradients.
 

Adenovirus Kit:

AdenoPACK 20
AdenoPACK 100
AdenoPACK 500
 

Adenovirus Downloads:

AdenoPACK 20, Technical Data Sheet
AdenoPACK 100, Technical Data Sheet
AdenoPACK 100RT, Technical Data Sheet
AdenoPACK 500, Technical Data Sheet
 

AdenoPACK 20

AdenoPACK 100

AdenoPACK 500

Available kit formats

Purification method

Process time

Eluate*

Recovery**

Viral Particles

AdenoPACK 20 / 20 ml culture

1 hour

1 ml

65-70%

1 x 10^11-12

AdenoPACK 100 / 60 ml culture

1-2 hours

1 ml

65%

1-3 x 10¹²

AdenoPACK 100 / 200 ml culture

2 hours

1 ml

80%

1 x 10¹³

AdenoPACK 100 RT ***/ 200 ml culture

2 hours

1 ml

80%

1 x 10¹³

AdenoPACK 500 / 500 ml culture

1-2 hours

1 ml

80%

1-3 x 10¹³

*after dialysis
**before buffer exchange
***kit does not contain Benzonase® Nuclease.

Benzonase® Nuclease is manufactured by Merck KGaA, Darmstadt, Germany and is covered by US Patent 5,173,418 and EP Patent 0,229,866. Nycomed Pharma A/S (Denmark) claims worldwide patent rights to Benzonase® Nuclease, which are licensed exclusively to Merck KGaA, Darmstadt, Germany. Benzonase® Nuclease is a registered trademark of Merck KGaA, Darmstadt, Germany.