Aleuria aurantia (AAL) (Biotin)

Cat# A1355-15-1mg

Size : 1mg

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Conjugate
Biotin

Grade
Molecular Biology Grade

Applications
E IHC WB

Shipping Temp
Blue Ice

Storage Temp
-20°C

mushroom
Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms.

Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.

Active Conjugate:
~95%

Lectin Biotinylated:
~90%

Inhibiting/Eluting Sugar:
100-400mM L-fucose

Sugar Specificity:
Fucose, Arabinose

Applications:
Suitable for use in Immunohistochemistry, Blots, ELISA and Cell Sorting. Other applications not tested.

Working Concentration:
5-20ug/ml

Recommended Buffers:
Immunohistochemistry: 10mM HEPES, pH 7.5, 0.15M sodium chloride.
Western Blot: 10mM Phosphate, pH 7.8, 0.15M sodium chloride, 0.1% Tween 20. Note: For chemiluminescence, concentrations are generally lower.
ELISA: 10mM Phosphate, pH 7.8, 0.15M sodium chloride.
Cell Sorting: 10mM HEPES pH 7.5, 0.15M sodium chloride.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source
Mushroom

Concentration
~2mg/ml

Form
Supplied as a liquid in HEPES, pH 7.5, 0.15M sodium chloride, 0.08% sodium azide. Labeled with Biotin.


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.