Notch Reporter BioAssay™ Kit (cAMP/PKA Pathway)

Cat# 171935-500T

Size : 500Tests

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Shipping Temp
Blue Ice

Storage Temp
-20°C

The Notch Pathway Reporter BioAssay™ kit is designed for monitoring the activity of the Notch signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for NOTCH1 that has a deletion of the entire extracellular domain (Notch1deltaE). Inside the cells, the NOTCH1 DE can be cleaved by upsilon-secretase and active NOTCH1 NICD is released into the nucleus. The kit also contains CSL (CBF1/RBP-Jk) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains the firefly luciferase gene under the control of multimerized CSL responsive elements upstream of a minimal promoter. The CSL (CBF1/RBP-Jk) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity.

Applications:
• Monitor Notch signaling pathway activity.
• Screen activators or inhibitors of Notch signaling pathway.
• Study effects of RNAi or gene overexpression on the activity of Notch pathway.

Kit Components:
171935A: Reporter, CSL (CBF1/RBP-Jkappa), 1x500ul
171935B: Negative Control, 1x500ul
171935C: Notch1∆E, 1x250ul
171935D: Negative Control Expression vector, 1x250ul

Note: These vectors are useful for transient transfection. They are not suitable for transformation and amplification in bacteria.

Storage and Stability:
Store all components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.