β-Glucosidase Assay Kit, BioAssay™

b-Glucosidase is a glucosidase enzyme which acts upon beta1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). b-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a b-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a b-glucosidase isoform (lysosomal glucocerebrosidase) has been related to Gaucher's disease and Parkinson's disease. This b-Glucosidase Assay Kit is designed to measure b-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-b-D-glucopyranoside that is hydrolyzed specifically by b-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.

High sensitivity and wide linear range. Use 20ul sample. The detection limit is 2U/L, linear up to 250U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of b-glucosidase activity within 20 minutes. Robust and amenable to high throughput analysis.

Direct Assays: b-glucosidase activity in biological samples. Characterization and Quality Control for b-glucosidase production. Drug Discovery: high-throughput screen for b-glucosidase modulators.

One unit of enzyme catalyzes the hydrolysis of 1mmole of substrate per min at pH 7.0.

G3059-11A: Assay Buffer, pH 7.0, 1x24ml
G3059-11B: b-NPG Substrate, 1x1ml
G3059-11C: Calibrator, 1x10ml (equivalent to 250 U/L)

Store all components at -20°C. Stable for 6 months after receipt.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.