Isocitrate Dehydrogenase BioAssay™ Kit (IDH)

Cat# 172375-100T

Size : 100Tests

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Shipping Temp
Blue Ice

Storage Temp
4°C/-20°C

Isocitrate Dehydrogenase (IDH) is an enzyme which catalyzes the interconversion of isocitrate and a-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD+ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP+ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the NADP+ isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. The non-radioactive, colorimetric IDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565nm. The increase in absorbance at 565nm is directly proportional to the enzyme activity.

Intended Use:
For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism.

Applications:
IDH activity determination in biological samples (e.g. plasma, serum, tissue and culture media.)

Range:
0.1-100U/L for 30 min reaction.

Detection limit:
0.1U/L

Kit Components:
Assay Buffer, 1x10ml
NADP/MTT, 1x1ml
Substrate, 1x1ml
Diaphorase, 1x120ul
Calibrator, 1x10ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.