QuantiChrom™ Isocitrate Dehydrogenase Assay Kit

Cat# DIDH-100

Size : 100tests

Brand : BioAssay Systems

Contact local distributor :


Phone : +1 850 650 7790

QuantiChrom™ Isocitrate Dehydrogenase Assay Kit



Application

  • For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism.

Key Features

  • Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 100 U/L for 30 min reaction.
  • Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • OD565nm

Samples

  • Plasma, serum, tissue and culture media, etc

Species

  • All

Procedure

  • 30 min

Size

  • 100 tests

Detection Limit

  • 0.1 U/L

Shelf Life

  • 6 months

More Details

ISOCITRATE DEHYDROGENASE (IDH) is an enzyme that catalyzes the interconversion of isocitrate and a-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD+ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP+ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the NADP+ isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. BioAssay Systems’ non-radioactive, colorimetric IDH assay is based on the reduction of the tetrazolium salt MTT in an NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

No citations for this new product. Please check back later. You may click here to check if citations are available, but are not listed here yet.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Isocitrate Dehydrogenase Assay Kit
Catalog No: DIDH-100 Categories Agriculture & Environment, Blood & Urine Chemistry, Enzyme Activity, Metabolism Tags Agriculture & Environment, Blood & Urine Chemistry, DIDH-100, Enzyme Activity, Isocitrate Dehydrogenase assay kits, Isocitrate Dehydrogenase determination kits, Isocitrate Dehydrogenase measurement kits, Isocitrate Dehydrogenase test kits, metabolism, QuantiChrom Isocitrate Dehydrogenase Assay Kits, QuantiChromTM Isocitrate Dehydrogenase Assay Kit