D-Lactate Assay Kit, BioAssay™, Colorimetric

Cat# L1011-04-96T

Size : 96Tests

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Shipping Temp
Dry Ice

Storage Temp
-20°C

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection.

Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. Lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565nm, is proportionate to the lactate concentration in the sample.

Key Features:
Sensitive and accurate: The detection limit of 0.05mM and linearity up to 2mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1mM and linearity up to 1mM D-lactate in a 96-well plate assay.

Convenient: The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 minutes. Room temperature assay. No 37°C heater is needed.

High-throughput: Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Application:
Direct Assays: D-lactate in serum, plasma, and cell media samples.

Kit Contents: (100 tests in 96-well plates):
L1011-04-A: Assay Buffer, 1x10ml
L1011-04-B: Enzyme A, 1x120ul
L1011-04-C: Enzyme B, 1x120ul
L1011-04-D: NAD Solution, 1x1ml
L1011-04-E: MTT Solution, 1x1.5ml
L1011-04-F: Standard (20mM D-Lactate), 1x1ml

Storage and Stability:
Store components at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.