FOXO Reporter BioAssay™ Kit (PI3K/AKT Signaling Pathway)

The FOXO Reporter BioAssay™ kit is designed to monitor activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cultured cells. The kit contains the transfection-ready FOXO3 expression vector and the FOXO luciferase reporter vector, which is a PI3K/Akt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimers of the FOXO responsive element located upstream of a minimal promoter. The FOXO reporter kit also contains several control vectors for transfection efficiency and background luminescence.

Reporter (Component A), FOXO luciferase reporter vector + constitutively expressing Renilla luciferase vector, 1x500ul (60ng DNA/ul)
Negative Control Reporter (Component B), Non-inducible luciferase vector + constitutively expressing Renilla luciferase vector, 1x500ul (60ng DNA/ul)
FOXO3 (Component C), Expression vector for FOXO3, 1x250ul (100ng DNA/ul)
Negative Control Expression Vector (Component D), Empty expression vector without FOXO3, 1x250ul (100ng DNA/ul)

Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.