Pyruvate Kinase BioAssay™ Kit (PK)

Cat# 172382-100T

Size : 100Tests

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Shipping Temp
Blue Ice

Storage Temp
4°C/-20°C

Pyruvate Kinase (PK) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Pyruvate kinase deficiency, a genetic disease, is caused by a lack of pyruvate kinase and slows down the process of glycolysis. Pyruvate kinase is also involved in gluconeogenesis, a biochemical pathway in which the liver generates glucose from pyruvate and other substrates. The Pyruvate Kinase Assay Kit provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity. In this assay PEP and ADP are catalyzed by pyruvate kinase to generate pyruvate and ATP. The color intensity of the reaction product at 570nm or fluorescence intensity at lex/em=530/590nm is directly proportional to
the pyruvate generated by the Pyruvate Kinase in the sample.

Intended Use:
For quantitative determination of pyruvate kinase activity and evaluation of drug effects on PK activity.

Applications:
Direct Assays: PK activity levels in plasma, serum, and tissue samples.
Drug Discovery/Pharmacology: effects of drugs on PK activity.

Range:
0.1-50U/L for colorimetric assays and 0.01-2U/L for fluorometric assays

Detection Limit:
0.01U/L

Kit Components:
Developer, 1x12ml
Dye Reagent, 1x120ul
Cosubstrate, 1x120ul
Pyruvate Standard, 1x400ul 25mM

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.