Sialic Acid Assay Kit, BioAssay™, Colorimetric/Fluorometric

Sialic Acid is a general name for nine carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The sialic acid level in body fluids is also an important marker for diagnosing cancer. Simple and direct procedures for measuring sialic acid concentrations find wide applications in research and drug discovery. Sialic acid assay uses an improved Warren method, in which sialic acid is oxidized to formylpyruvic acid which reacts with thiobarbituric acid to form a pink colored product. The color intensity at 549nm or fluorescence intensity at λem/λex=585/555nm is directly proportional to sialic acid concentration in the sample.

Sensitive and Accurate:
•Use as little as 60ul samples
•Linear detection range in 96-well plate
•5-1000uM sialic acid for Colorimetric Assays
•0.5-100uM for Fluorometric Assays

Direct Assays: Sialic acid in biological samples (e.g. serum, plasma, saliva, milk)

S1013-31P1: Dye Reagent, 1x6ml
S1013-31P2: TCA, 10%, 1x5ml
S1013-31P3: DMSO, 1x12ml
S1013-31P4: Oxidation Reagent, 1x10ml
S1013-31P5: Hydrolysis Reagent, 1x10ml
S1013-31P6: Standard (10mM Sialic acid), 1x500ul

Store *S1013-31P6 at -20°C. Store other components at RT. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.