PTN Chemi-Luminescent ELISA Kit (Bovine)
Cat# OKCD03983
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Printable datasheet for PTN Chemi-Luminescent ELISA Kit (Bovine) (OKCD03983) |
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Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Pleiotrophin (PTN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pleiotrophin (PTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pleiotrophin (PTN) level in the sample or standard. | ||||||||||||||||||||||
ELISA Kit Range | 2.74-2,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 31.2 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | PTN |
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Gene Full Name | pleiotrophin |
Alias Symbols | HBBM;HB-GAM;HBGF-8;heparin binding growth factor 8;heparin-binding brain mitogen;Heparin-binding growth factor 8;heparin-binding growth-associated molecule;heparin-binding neurite-promoting factor;neurite growth-promoting factor 1;p18;pleiotrophin;pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1). |
NCBI Gene Id | 280904 |
Protein Name | Pleiotrophin |
Description of Target | Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells (PubMed:1550956). Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. Binds to cell-surface target proteins via their chondroitin sulfate groups. Down-regulates PTPRZ1 activity. |
Uniprot ID | P21782 |
Protein Accession # | NP_776380 |
Nucleotide Accession # | NM_173955 |