SuperFast NGS DNA Library Preparation Kits for Illumina

SuperFast NGS DNA Library Preparation Kits for Illumina

Enzymatique process of "tagmentation" for saving time

Total time: 1.5 hours

1. Tagmentation of DNA
 DNA is fragmented by an enzyme called tagmentase. The tagmentatase is a modified transposase: a mixture of a transposase and a transposon end complex that forms the transposome. Tagmentation consists of DNA fragmentation and the simultaneous addition of Illumina® sequencing adapters.
Tagmentation technology eliminates the various purification and ligation steps required for the conventional method.
We offer different kits for which the tagmentation step has been optimized for different amounts of initial DNA (50 ng, 5 ng and 1 ng).

2. Amplification by cycle-limited PCR
In this second step, the fragmented DNA is amplified by cycle-limited PCR, using primers targeted to the transposon sequence. These primers add the specific adapters and barcodes (if desired) to the fragments.

Tagmentation technology therefore significantly reduces library preparation time and the initial amount of DNA.

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