Optimum Growth 2.8L Flask w/ Vent Cap, Sterile

Cat# 931114

Size : CS6

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Designed for mammalian and insect cell culture, superior to traditional shake flasks making shake cabinets a much more efficient space in the lab.

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Shake Flasks

Designed for mammalian and insect cell culture, superior to traditional shake flasks making shake cabinets a much more efficient space in the lab.

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Special Flasks

Thomson Optimum Growth® Special Flasks were designed with the unique needs of small-to-medium-scale bioprocessing applications

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Transfer Caps

Eliminates intermediate vessels for scale up & seed cultures

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Rapid Clear® Cap

Quickly and efficiently clarify cell culture media directly from the Thomson 5L Optimum Growth® shaker flask without the need for centrifugation

Scalable from 125mL up to 5L

Thomson Optimum Growth® Flasks (patented) for mammalian and insect cell culture come in multiple sizes of 125mL, 250mL, 500mL, 1.6L, 2.8L and our popular 5L.They are superior to traditional shake flasks due in part to the fact that they support a 40-50% fill volume versus 33% fill volume making your shake cabinet a much more efficient space.

  • Baffles designed for high aeration and low shear to maintain cell viability

  • Same footprint as comparable Fernbach flask but with a 40-50% fill volume

  • Less foaming than disposable Fernbach potentially eliminates additives

  • 0.2μm Vented Cap simultaneously maintains high gas exchange and sterility

  • Transfer cap option connects directly to cell bags or bioreactors with multiple connection options

  • Scalability from 125mL up to 5L lets you mix and match different flask sizes in your shaker greatly improving space efficiency

  • Individually packaged and sterilized for immediate use

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Maximize Shaker Space Efficiency

Thomson Optimum Growth® Flasks in all sizes shaking at an optimal shake speed of 140 rpm on a 1" (2.54cm) throw shake platform.

Note: that virtually every square inch of platform space is utilized

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Optimum Growth® Flasks give excellent growth with space saving capability.
See Optimum Growth® Accessories
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Optimum Growth® Best Working Volume 40-50%

Working fill volume & shake speeds for Optimum Growth® Flasks for the best advertised results.

Flask SizeFill VolumeWorking RPM Range 1" (2.54cm) | 2" (5.08cm)Vol./Size Ratio
125mL-LE*15-40mL100-120 | 9012-32%
125mL50-63mL140-150 | 11040-50%
250mL100-125mL140-150 | 11040-50%
500mL200-250mL140-150 | 11040-50%
1.6L400-900mL140-150 | 11040-50%
2.8L1.0-1.4L140-150 | 11040-50%
5L2.0-2.5L140-150 | 11040-50%

*LE = Low Evaporation for small volume applications

Comparison of Two Expressed Membrane Proteins
  • Corning® - 500mL flask, 200mL culture
  • Thomson - 250mL flask, 150mL culture
  • 4mL samples purified over Ni-NTA
  • Protein A - Membrane protein of moderate expression, 34kDa
  • Protein B - Membrane protein of low expression, 45kDa
  • 12μL of elution resolved on a coomassie gel

Conclusion: Thomson Optimum Growth® flasks are equivalent to Corning® standard flasks in terms of expressed protein purity but with a 50-60% fill volume Optimum Growth® flasks generate a far greater total yield/flask.

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Protein Production/Flask

Data supplied by New York Structural Genomics Research Consortium

Four insect cell line clones cultured in Thomson 5L Optimum Growth® flasks and Corning 3L shake flasks. In the figure below note the consistently higher protein yield with Optimum Growth® flasks over Corning. Each flask has the same footprint but flasks operate with 3x higher fill volume (illustrated in the figure at right).
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Thomson 5L flasks consistently maximize production of your best expressers
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4-20% SDS-PAGE Quick Blue Stain Commassie Gel Expected MW of dimer 24.5 kDa Estimated expression level ~10-20 mg/L

Low Expressing Gel

This gel shows equal bands from 5 replicates of a low expressing protein, producing roughly 10 to 20 mg/L.

Gel Key
  1. Benchmark Pre-Stained Protein Ladder
  2. Purified protein, 100 ng control
  3. Purified protein, 200ng control
  4. Untransfected cells, -ve control
  5. +ve control
  6. +ve control
  7. Protein of interest, 5L Combined Flasks #1-5
  8. Protein of interest, 5L Flask #1
  9. Protein of interest, 5L Flask #2
  10. Protein of interest, 5L Flask #3
  11. Protein of interest, 5L Flask #4
  12. Protein of interest, 5L Flask #5
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4-20% SDS-PAGE Quick Blue Stain Commassie Gel Expected MW of dimer 159.4 kDa Estimated expression level ~300 mg/L

High Expressing Gel

This gel shows equal bands from 3 replicates of a high expressing protein, producing approximately 300 mg/L.

Gel Key
  1. Benchmark Pre-Stained Protein Ladder
  2. Purified mAb 100 ng control
  3. Purified mAb 250 ng control
  4. Purified mAb 500 ng control
  5. Purified mAb 1000 ng control
  6. +ve control
  7. Protein of interest, 5L Flask #1
  8. Protein of interest, 5L Flask #2
  9. Protein of interest, 5L Flask #3
  10. Protein of interest, 5L Combined Flasks #1-3

Conclusion

Thomson Optimum Growth® Flasks not only ensure consistent expression from HEK293 strains, they can also increase shaker capacity.

With the same footprint as a typical Corning® 3L flask and a culture volume of up to 3L, the Optimum Growth® 5L Flask may increase production 200%, if not more, in the same space (this is construct dependent).

Most constructs express at higher levels in the Optimum Growth® 5L flasks. This makes one Optimum Growth® 5L equivalent to, if not greater than, two 3L flasks.

Thomson Instrument Company is not affiliated with Corning® Life Sciences or their products

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Refer to the chart below to find the appropriate flask clamp compatible with your shaker.

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