A multi-component enzyme system (1). Although the preparation has high cellulase activity (EC 3.2.1.4), it still contains hemicellulases, and it degrades mannans, xylans, galactomannans, pectins and other polysaccharides. Contains about three times as high xylanase activity as Cellulase Onozuka R-10^® (cat. no. 16419). Widely used for the isolation of protoplasts, for its ability to degrade cell walls, often in combination with Macerozyme R-10^® (cat. no. 28302) (2).
Temperature optimum: 50 - 60 °C
pH-optimum: pH 4 - 5

Unit definition: 1 U catalyzes the liberation of 1 µmole glucose from sodium carboxymethyl cellulose per minute at 40 °C, pH 4.5; glucose determined with alkaline copper reagent (3).
Extraneous activities: Contains α-amylase, pectinase, protease and hemicellulase.
Onozuka R-10^® is a registered trademark of Yakult Pharmaceuticals Industry Co., Ltd..


References:

1. Beldman, G. et al. (1985) Eur. J. Biochem. 146, 301-8
2. Potrykus, J. & Shillito, R.D. (1986) Methods Enzymol. 118, 549-78
3. Okada, G. (1988) Methods Enzymol. Vol. 160, 259-63
4. Lendl & Bauer (1989) Zell- und Gewebekultur, Gustav Fischer Verlag, 147ff.

DANGER

Hazard Statements	H334
Precautions	P261 - P285
Reaction	P304 +P341 - P342 +P311

EG-Index: 647-002-00-3 • EINECS: 232-734-4 • WGK: 1 • HS: 35079090
Storage Temperature: +2 °C to +8 °C