- More Files
- Specification
Product Description
Mouse monoclonal antibody raised against recombinant VEGFA.
Immunogen
Recombinant protein corresponding to human VEGFA.
Host
Mouse
Reactivity
Dog, Human, Mouse, Rat
Specificity
This is specific to a cluster of proteins between 19 and 22kD (reduced).
Form
Liquid
Isotype
IgG1, kappa
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Western Blot (Tissue lysate)
Western blot analysis of VEGFA in human kidney protein using VEGFA monoclonal antibody, clone VG1 (Cat # MAB6996).Immunohistochemistry
Immunofluorescence
- Gene Info — VEGFA
Entrez GeneID
7422Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
- Interactome
- Pathway
- Disease
- Publication Reference
- Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells.
Ptaszynska MM, Pendrak ML, Bandle RW, Stracke ML, Roberts DD.
Molecular Cancer Research: MCR 2008 Mar; 6(3):352.
Application:Func, Human, SKOV3 cells.
- DEC1 (STRA13) protein expression relates to hypoxia- inducible factor 1-alpha and carbonic anhydrase-9 overexpression in non-small cell lung cancer.
Giatromanolaki A, Koukourakis MI, Sivridis E, Turley H, Wykoff CC, Gatter KC, Harris AL.
The Journal of Pathology 2003 Jun; 200(2):222.
Application:IHC-P, Human, Non-small cell lung cancer.
- Expression of VEGF in routinely fixed material using a new monoclonal antibody VG1.
Turley H, Scott PA, Watts VM, Bicknell R, Harris AL, Gatter KC.
The Journal of Pathology 1998 Nov; 186(3):313.
Application:IHC-P, WB, Human, Monkey, COS cells, Small capillary, Ovary, Bone marrow, Renal glomerular capillaries, Endometrial gland cells, Breast duct cells, Placenta, Lung, Squamous cell carcinoma, Melanoma.
- Positive feedback between vascular endothelial growth factor-A and autotaxin in ovarian cancer cells.