ALDH2 Protein
Cat# OPPA02181
Size : 10ug
Brand : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for OPPA02181 |
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| Predicted Species Reactivity | Human |
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| Product Format | ALDH2 protein contains 20mM Tris-buffer, pH-7.5, 1mM DTT, 1mM EDTA and 10% Glycerol. Physical appearance: Sterile Filtered clear solution. |
| Host | E. Coli |
| :: | Biological Activity: Specific activity is < 0.2 units/ml, and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25C in the presence of beta-NAD, potassium and thiols. |
| :: | Product Introduction: ALDH2 is part of the aldehyde dehydrogenase family of proteins which catalyze the chemical transformation from acetaldehyde to acetic acid. ALDH2 is the second enzyme of the major oxidative pathway of alcohol metabolism. ALDH2 has 2 major liver isoforms: cytosolic and mitochondrial, which differ by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Nearly all Caucasians have 2 major isozymes, whereas roughly 50% of Orientals have only the cytosolic isozyme, omitting the mitochondrial isozyme. The extremely higher rate of acute alcohol intoxication with Orientals compared to Caucasians is due to the fact of the absence of mitochondrial isozyme. ALDH2 has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Product Description: ALDH2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 501 amino acids (18-517 a.a.) & having a molecular mass of 54.5 kDa. The ALDH2 is purified by proprietary chromatographic techniques. |
| Reconstitution and Storage | Store vial at -20C to -80C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Peptide Sequence | MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLNRLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAGTVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S. |
| Gene Symbol | ALDH2 |
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| Alias Symbols | ALDM, ALDHI, ALDH-E2 |
| NCBI Gene Id | 217 |
| Protein Name | Aldehyde dehydrogenase, mitochondrial |
| Description of Target | Recombinant Human Aldehyde Dehydrogenase 2 |
| Uniprot ID | P05091 |
| Protein Accession # | NP_000681.2 |
| Protein Size (# AA) | Recombinant |