BOVINE SERUM ALBUMIN - Fraction V (Immunoglobulin and Protease Free)
Cat# OORA01664
Size : 50g
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Product Format | Lyophilized |
|---|---|
| Conjugation | Unconjugated |
| Application | Enzyme-linked immunosorbent assay, Flow cytometry, Fluorescence-linked immunosorbent assay, Functional assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot |
| Additional Information | Moisture: 5% loss on drying pH: 7.0 Ash: < 2.0% Protein: 98% (nitrogen analysis) Protease: < 0.005 Units/mg Endotoxin: < 3 EU/mg by LAL IgG: not detected NEFA: N/A |
| Reconstitution and Storage | Restore with deionized water (or equivalent). Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of receipt. |
| Purity | 100% (Agarose Zone electrophoresis) |
| Application Info | Bovine Serum Albumin - Fraction V is suitable for use in lateral flow, in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding for FACS, IF, IHC, and WB. |
| Alias Symbols | BSA Blocker, BSA Blocking |
|---|---|
| Description of Target | Bovine Serum Albumin (BSA) is used for various biochemical applications including ELISA (Enzyme-Linked Immunosorbent Assay), high content screenining assays, western blotting, FACS Buffer and immunohistochemistry. BSA as a blocking reagent is particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Bovine Serum Albumin can also be used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. |