Goat IgG Human IgG IgA IgM (HRP) antibody

Cat# orb21831-1ml

Size : 1ml

Brand : Biorbyt

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Goat IgG Human IgG IgA IgM (HRP) antibody

Goat IgG Human IgG IgA IgM (HRP) antibody

Catalog Number: orb21831

Catalog Numberorb21831
CategoryAntibodies
DescriptionHorseradish peroxidase-conjugated IgG fraction of polyclonal goat antiserum to human IgG, IgA and IgM, heavy and light chains. In enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:1,500 and 1:15,000.
Species/HostGoat
ClonalityPolyclonal
ReactivityHuman
Form/AppearancePeroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
ConjugationHRP
Hazard InformationThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
SourcePurified polyclonal human IgG, and IgA and IgM containing factions isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
StorageStorage: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate
Buffer/PreservativesPeroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.
NoteFor research use only
Expiration Date12 months from date of receipt.