Pisum sativum (PSA/PSL) (AP)

Cat# 518666-1mg

Size : 1mg

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Conjugate
AP

Grade
Highly Purified

Shipping Temp
Blue Ice

Storage Temp
-20°C

PSA/PSL
Alkaline phosphatase (AP) is conjugated to Pisum sativum Lectin (PSA/PSL) to show the binding of PSA/PSL in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140kD) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Source:
Pea

Blood Specificity:
Non-specific

Sugar Specificity:
alpha-Mannose, alpha-Glucose

Inhibitory Carbohydrate:
alpha-Methylmannoside + alpha-Methylglucoside

Divalent Ions:
Ca++, Mn++

Storage and Stability:
Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 6 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Source
Pea

Purity
Highly Purified

Form
Supplied as a liquid in 10mM HEPES, 150mM NaCl, 0.1mM Ions, 0.02% sodium azide. Labeled with Alkaline Phophatase (AP).


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.