Caspase mediated apoptosis assay kits
Analyze caspase activation in living cells by flow cytometry or fluorescence microscopy.
Analyze caspase activity in cell lysate using fluorometer or fluorescence plate reader (Ex/Em = 400/505 nm).
Analyze caspase activity in cell lysate using spectrophotometer or plate reader (O.D. = 400 nm).
Effectively screen caspase inhibitors using fluorescence-based method (Ex/Em = 400/505 nm).
Quantify the specific activity of caspase-3 or -7, respectively using fluoresence-based method (Ex/Em = 400/505 nm). Other caspases and non-specific proteases are not detected.
Detecting activation of caspases by flow cytometry in living cells (Ex/Em = 488/530 nm).
Detection of Caspase-3 Activation Using CaspGLOW Fluorescein Caspase-3 Staining Kit.
Apoptosis was induced in Jurkat cells with (B) or without (A) camptothecin for 6 hours. Cells were collected and incubated with CaspGLOW in situ marker, FITC-DEVD-FMK, for 20 minutes according to the kit instructions. Results were analyzed by flow cytometry.