New Ultra-Sensitive qPCR Gene Mutation Detection Kits

New Ultra-Sensitive qPCR Gene Mutation Detection Kits

QClamp® Detects Rare and Actionable Cancer Mutations & Fusion Genes with ultrasensitivity
New Technology : Xenonucleic Acid (XNA) Molecular Clamp Technology

QClamp® Gene Mutation Detection Kit is a highly sensitive qPCR assay that provides detection sensitivity of 0.1% or lower. It is ideal for screening rare and actionable somatic mutations in oncogenes. The assay utilizes a sequence-specific clamp (Xeno-Nucleic Acid probe) that suppresses PCR amplification of wild-type DNA template and selectively amplifies only mutant template. The kits provide a rapid, reproducible and affordable solution which employs a simple workflow and PCR machines that are commonly used in research and clinical labs.

Ability to detect below 0.1% mutations 
Suitable for liquid biopsy, FFPE tissue and more 
Streamlined workflow with 3 hours turnaround time 
Turnaround time ~ 2 hours 
Codons 12, 13, 59, 61, 117, & 146 
Codons 12, 13, 59, 61, 117 & 146
Codons 719, Ex19Del, 790, 858 & 861
Codon 600
Codons 542, 545 & 1047
Codon 617
Codon 816

Xenonucleic Acid (XNA) Molecular Clamp Technology

DiaCarta scientists have developed innovative new nucleic acid molecular oligomers that hybridize by Watson-Crick base pairing to target DNA sequences yet have a modified chemical backbone. The xenonucleic acid oligomers (figure: Watson-Crick Base Pairing of DNA with cognate XNA) are highly effective at hybridizing to target sequences and can be employed as molecular clamps in quantitative real-time polymerase chain reactions (PCR) or as highly specific molecular probes for detection of nucleic acid target sequences.


  • Molecular clamps for qPCR
  • Synthetic oligomers containing natural A, T,C, G or modified nucleosides (15 to 25 nt long)
  • Hydrophilic and neutral backbone (no phosphate group like PNA)
  • Hybridization by Watson-Crick pairing
  • Resistant to any known nucleases
  • Much higher binding affinity
  • DNA binding is independent of salt concentration
  • Large melting temperature differential (ΔTm = 15-20ºC) in single-nucleotide (SNP's) and insertion/deletions (indels) (5-7ºC for natural DNA)

How it works

QClamp® is a revolutionary new way to screen for somatic mutations that utilize a sequence-specific clamp that suppresses PCR amplification of wild type template DNA. QClamp® allows selective PCR amplification of only mutant templates, which allows the detection of mutant DNA in the presence of a large excess of wild-type templates from a variety of samples including FFPE, liquid biopsy, and traditionally challenging cytology samples.

Supporting data

QClamp Detects below 0.1% mutated DNA
The most sensitive technology that could detect below 0.1% mutant in 2 hours.

QClamp HRM for EGFR Mutation
High-resolution melting (HRM) profiles of clinically relevant EGFR mutations generated by the QClamp EGFR Test.