ZyBlack™ Quenching solution for FISH

ZyBlack™ Quenching solution for FISH

Reduce autofluorescence on in situ hybridization


ZyBlack Quenching solution reduces autofluorescence

One of the major concerns of Fluorescence in situ Hybridization (FISH)-based diagnostic assays is the interference by autofluorescence. Several types of tissue tend to emit intense autofluorescence, including brain, liver, kidney and myocardium, making it difficult to evaluate FISH results.
ZyBlack Quenching Solution is a ready-to-use solution to reduce autofluorescence on both formaldehyde-fixed paraffin-embedded and frozen sections. It can be easily incorporated into the FISH protocol by applying it after the proteolytic pretreatment.
ZyBlack Quenching Solution reduces autofluorescence without adversely affecting tissue integrity or specific fluorescence signals.

Advantages :
  • Ready-to-use
  • For FFPE and frozen tissue sections
  • Easy to incorporate into FISH protocol : apply it after the proteolytic pretreatment
  • CE/IVD marked
  • Compatible ZytoLight and FlexISH probes
Tissues with high autofluorescence :

  • Brain
  • Liver
  • Kidney
  • Myocardium

Without ZyBlack Quenching Solution

With ZyBlack Quenching Solution


Brain tissue section hybridized with the ZytoLight SPEC 1p36/1q25 Dual Color Probe 


Product code Size Tests
BS-0002-8 8 ml 20 tests

Find all in situ hybridization probes HERE