ZyBlack™ Quenching solution for FISH
Reduce autofluorescence on in situ hybridization
ZyBlack Quenching solution reduces autofluorescence
One of the major concerns of Fluorescence in situ Hybridization (FISH)-based diagnostic assays is the interference by autofluorescence. Several types of tissue tend to emit intense autofluorescence, including brain, liver, kidney and myocardium, making it difficult to evaluate FISH results.
ZyBlack Quenching Solution is a ready-to-use solution to reduce autofluorescence on both formaldehyde-fixed paraffin-embedded and frozen sections. It can be easily incorporated into the FISH protocol by applying it after the proteolytic pretreatment.
ZyBlack Quenching Solution reduces autofluorescence without adversely affecting tissue integrity or specific fluorescence signals.
Tissues with high autofluorescence :
Without ZyBlack Quenching Solution
With ZyBlack Quenching Solution
Brain tissue section hybridized with the ZytoLight SPEC 1p36/1q25 Dual Color Probe
|BS-0002-8||8 ml||20 tests|
Find all in situ hybridization probes HERE