New Ultra-Sensitive qPCR Gene Mutation Detection Kits
QClamp® Detects Rare and Actionable Cancer Mutations & Fusion Genes with ultrasensitivity
New Technology : Xenonucleic Acid (XNA) Molecular Clamp Technology
QClamp® Gene Mutation Detection Kit is a highly sensitive qPCR assay that provides detection sensitivity of 0.1% or lower. It is ideal for screening rare and actionable somatic mutations in oncogenes. The assay utilizes a sequence-speciﬁc clamp (Xeno-Nucleic Acid probe) that suppresses PCR ampliﬁcation of wild-type DNA template and selectively ampliﬁes only mutant template. The kits provide a rapid, reproducible and aﬀordable solution which employs a simple workﬂow and PCR machines that are commonly used in research and clinical labs.
Ability to detect below 0.1% mutations
Suitable for liquid biopsy, FFPE tissue and more
Streamlined workflow with 3 hours turnaround time
Turnaround time ~ 2 hours
Codons 12, 13, 59, 61, 117, & 146
Codons 12, 13, 59, 61, 117 & 146
Codons 719, Ex19Del, 790, 858 & 861
Codons 542, 545 & 1047
Xenonucleic Acid (XNA) Molecular Clamp Technology
DiaCarta scientists have developed innovative new nucleic acid molecular oligomers that hybridize by Watson-Crick base pairing to target DNA sequences yet have a modified chemical backbone. The xenonucleic acid oligomers (figure: Watson-Crick Base Pairing of DNA with cognate XNA) are highly effective at hybridizing to target sequences and can be employed as molecular clamps in quantitative real-time polymerase chain reactions (PCR) or as highly specific molecular probes for detection of nucleic acid target sequences.
FEATURES AND BENEFITS
How it works
QClamp® is a revolutionary new way to screen for somatic mutations that utilize a sequence-specific clamp that suppresses PCR amplification of wild type template DNA. QClamp® allows selective PCR amplification of only mutant templates, which allows the detection of mutant DNA in the presence of a large excess of wild-type templates from a variety of samples including FFPE, liquid biopsy, and traditionally challenging cytology samples.
QClamp Detects below 0.1% mutated DNA
The most sensitive technology that could detect below 0.1% mutant in 2 hours.
QClamp HRM for EGFR Mutation
High-resolution melting (HRM) profiles of clinically relevant EGFR mutations generated by the QClamp EGFR Test.