New Ultra-Sensitive qPCR Gene Mutation Detection Kits
QClamp® Detects Rare and Actionable Cancer Mutations & Fusion Genes with ultrasensitivity
New Technology : Xenonucleic Acid (XNA) Molecular Clamp Technology
QClamp® Gene Mutation Detection Kit is a highly sensitive qPCR assay that provides detection sensitivity of 0.1% or lower. It is ideal for screening rare and actionable somatic mutations in oncogenes. The assay utilizes a sequence-speciﬁc clamp (Xeno-Nucleic Acid probe) that suppresses PCR ampliﬁcation of wild-type DNA template and selectively ampliﬁes only mutant template. The kits provide a rapid, reproducible and aﬀordable solution which employs a simple workﬂow and PCR machines that are commonly used in research and clinical labs.
Ability to detect below 0.1% mutations
Suitable for liquid biopsy, FFPE tissue and more
Streamlined workflow with 3 hours turnaround time
Codons 12, 13, 59, 61, 117, & 146
Codons 12, 13, 59, 61, 117 & 146
Codons 719, Ex19Del, 790, 858 & 861
Codons 542, 545 & 1047
Medical devices for in vitro diagnostic. Read the instructions for use carefully.
Xenonucleic Acid (XNA) Molecular Clamp Technology
DiaCarta scientists have developed innovative new nucleic acid molecular oligomers that hybridize by Watson-Crick base pairing to target DNA sequences yet have a modified chemical backbone. The xenonucleic acid oligomers (figure: Watson-Crick Base Pairing of DNA with cognate XNA) are highly effective at hybridizing to target sequences and can be employed as molecular clamps in quantitative real-time polymerase chain reactions (PCR) or as highly specific molecular probes for detection of nucleic acid target sequences.
FEATURES AND BENEFITS
How it works
QClamp® is a revolutionary new way to screen for somatic mutations that utilize a sequence-specific clamp that suppresses PCR amplification of wild type template DNA. QClamp® allows selective PCR amplification of only mutant templates, which allows the detection of mutant DNA in the presence of a large excess of wild-type templates from a variety of samples including FFPE, liquid biopsy, and traditionally challenging cytology samples.
QClamp Detects below 0.1% mutated DNA
The most sensitive technology that could detect below 0.1% mutant in 2 hours.
QClamp HRM for EGFR Mutation
High-resolution melting (HRM) profiles of clinically relevant EGFR mutations generated by the QClamp EGFR Test.