Custom service: Teratoma Formation Assay
A gold standard method to characterize pluripotency of human and mouse ES/iPS cells.
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- Fast turnaround time--as short as four weeks.
Teratomas formed after subcutaneous injection often contain undefined tissues that are difficult to identify (A-C). In contrast, injection under the kidney or testis capsule results in characteristic teratomas with differentiated tissues derived from all three germ layers (D-I).
Only ~60%of human iPS cell lines form teratomas in both kidney and testis. The other lines preferentially form teratomas in either one, but not in both organ types. Therefore, we always inject into both the kidney and testis capsule (four injection sites total).